Glucose catabolism via a partially cyclic pentose phosphate pathway in Gluconobacter 1 oxydans 621 H : a combined fluxomics and transcriptomics analysis

نویسندگان

  • Tanja Hanke
  • Katharina Nöh
  • Stephan Noack
  • Tino Polen
  • Stephanie Bringer
  • Wolfgang Wiechert
  • Michael Bott
چکیده

Glucose catabolism via a partially cyclic pentose phosphate pathway in Gluconobacter 1 oxydans 621H: a combined fluxomics and transcriptomics analysis 2 3 Tanja Hanke, Katharina Nöh,* Stephan Noack, Tino Polen, Stephanie Bringer, Hermann 4 Sahm, Wolfgang Wiechert, Michael Bott* 5 Institut für Biound Geowissenschaften, IBG-1: Biotechnologie, Forschungszentrum Jülich, 6 D-52425 Jülich, Germany 7 8 9

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Glucose catabolism via a partially cyclic pentose phosphate pathway in Gluconobacter 1 oxydans 621H: a combined fluxomics and transcriptomics analysis 2 3

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Glucose catabolism via a partially cyclic pentose phosphate pathway in Gluconobacter

Glucose catabolism via a partially cyclic pentose phosphate pathway in Gluconobacter 1 oxydans 621H: a combined fluxomics and transcriptomics analysis 2 3 Tanja Hanke, Katharina Nöh,* Stephan Noack, Tino Polen, Stephanie Bringer, Hermann 4 Sahm, Wolfgang Wiechert, Michael Bott* 5 Institut für Biound Geowissenschaften, IBG-1: Biotechnologie, Forschungszentrum Jülich, 6 D-52425 Jülich, Germany 7 8 9

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Combined fluxomics and transcriptomics analysis of glucose catabolism via a partially cyclic pentose phosphate pathway in Gluconobacter oxydans 621H.

In this study, the distribution and regulation of periplasmic and cytoplasmic carbon fluxes in Gluconobacter oxydans 621H with glucose were studied by (13)C-based metabolic flux analysis ((13)C-MFA) in combination with transcriptomics and enzyme assays. For (13)C-MFA, cells were cultivated with specifically (13)C-labeled glucose, and intracellular metabolites were analyzed for their labeling pa...

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13C Tracers for Glucose Degrading Pathway Discrimination in Gluconobacter oxydans 621H

Gluconobacter oxydans 621H is used as an industrial production organism due to its exceptional ability to incompletely oxidize a great variety of carbohydrates in the periplasm. With glucose as the carbon source, up to 90% of the initial concentration is oxidized periplasmatically to gluconate and ketogluconates. Growth on glucose is biphasic and intracellular sugar catabolism proceeds via the ...

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Microorganisms capable of producing xy]itol from Darabitol were screened for. Of the 420 strains tested, three bacteria, belonging to the genera Acetobacter and Gluconobacter, produced xylitol from D-arabitol when intact cells were used as the enzyme source. Among them, Gluconobacter oxydans ATCC 621 produced 29.2 g!1 xylitol from 52.4 g!1 D-arabitol after incubation for 27 h. The production of...

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تاریخ انتشار 2013